Olympus BX43 Instructions Manual

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INSTRUCTIONS BX43 SYSTEM MICROSCOPE This instruction manual is for the Olympus System Microscope Model BX43. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you st...

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In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olymp...

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BX43 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 10, “ASSEMBLY” (pages 33 to 37) carefully. IMPORTANT -- Be...

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5-6 Immersion Objectives ................................................................................................................................................................. 25 1 Using Immersion Objectives ................. 25 5-7 O...

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1 BX43 Fig. 1 SAFETY PRECAUTIONS (Fig. 1) IMPORTANT 1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection, clean the parts coming in contact with the specimen to prevent infection. ...

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2 Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol Explanation Indicates that the surface becomes hot, and should not be touched wi...

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3 BX43 2 Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerpr...

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4 1 MODULE NOMENCLATURE } The modules mentioned below show only the typical product names. As there are some products that are not mentioned but also applicable to this microscope, check the latest catalogues or consult Olympus. For the products ...

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5 BX43 2 CONTROLS } If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 33 to 37). Interpupillary distance adjustment scale (Page 22) Diopter adjustment ring (Page 22) Slide holder clamping lever (Page 19) Aperture ...

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6 Main switch (Page 10) I : ON : OFF Fine adjustment knob (Page 16) Pre-focusing lever (Page 16) LIM indicator ON: Lit (Green) OFF: Extinguished LIM ON-OFF switch (Page 13) LED brightness adjustment knob (Page 13) LIM setting switch (Page 13) ...

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7 BX43 Low Magnification Condenser U-LC LS30 Adapter U-LS30ADP Swing-out Condenser U-SC3 Halogen Lamp Socket U-LS30-5 Auxiliary lens mounting tool (for BX53) Auxiliary lens * The auxiliary lens is mounted on the lamp adapter when the U-LC is used....

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8 3 FLOW OF OBSERVATION } When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated. } When the halogen bulb is used, set the LBD filter in the filter mount on the base (or in the U-FC filter cassette). (Controls U...

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9 BX43 } Copy the observation procedure pages on separate sheets and post it near your microscope. 9 a 5 (trinocular tube only) d c e 2 f 3 7 1 4 b 8 6 g ...

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10 SIMPLIFIED OBSERVATION PROCEDURE 4 4-1 Basic Operation (Until Observation of Specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each con...

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11 BX43 Fig. 7 Fig. 6 Fig. 8 5 6 7 1 1 2 Rotate the revolving nosepiece to engage the 10X objective in the light path. Rotate the coarse and fine adjustment knobs to bring the specimen into focus. (Details: Page 17) Rotate the stage knob to adjust...

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12 3 Adjusting the Centering Place the specimen. Rotate the revolving nosepiece to select the 10X objective. Rotate the knobs to bring the specimen into focus. Rotate the knob to raise the condenser to its upper limit. Rotate the field iris diaph...

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13 BX43 5 USING THE CONTROLS 5-1 Base Fig. 10 Fig. 11 Fig. 12 1 Adjusting the Brightness (Fig. 10) 1. When the LED lamp is used for observation, turn the LED brightness adjustment knob @ clockwise to make illumination brighter. 2. Wh...

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14 Fig. 14 Fig. 15 Using the Filter Cassette (Figs. 14 to 17) Loading Filters into Filter Cassette } The filter cassette accommodates filters with a diameter of 45 mm and thickness of 2.7 mm or less. } The filter cassette has two filter levels on...

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15 BX43 Fig. 16 Fig. 17 Using the Filter Cassette (Fig. 17) Usable Filters Applications 45LBD-IF* Color balancing filter 45-ND6, 45-ND25 Neutral density filter 45G-530, 43IF550-W45* Green B&W contrast filters 45O-560 Orange Up to three of t...

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16 Fig. 18 Fig. 19 Fig. 20 5-2 Focusing Block 1 Focusing Controls The focusing block consists of the controls listed in the following table, which also describes the functions of the controls. No. Name Function 1 Coarse adjustment knob Moves...

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17 BX43 Fig. 21 Fig. 22 Fig. 23 3 Replacing the Fine Adjustment Knob (Fig. 21) The fine adjustment knob has been attached on the right side at the factory. } The fine adjustment knob is designed detachable to prevent interference with hand d...

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18 Fig. 24 Fig. 25 Fig. 26 5-3 Stage 1 Placing the Specimen } The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. } When observing very large specimens, r...

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19 BX43 3 Adjusting the X- and Y-Axis Knob Tension (Fig. 27) 1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob | clockwise (...

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20 4 Rotating the Stage (Fig. 28) 1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. A click may be heard and felt during rotation. However, this is due...

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21 BX43 Fig. 31 Fig. 32 5-4 Observation Tube 1 Adjusting the Diopter When diopter adjustment rings are provided on both the left and right, it is possible to adjust the optimum vision for both the left and right eyes as described below. The di...

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22 3 Using the Eyepiece Micrometer Disk (Fig. 33) When the WHN10X-H (WHN10X) eyepieces are used, an eyepiece micrometer disk can be inserted in one of them. When the eyepiece does not have a diopter adjustment mechanism, however, it ...

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23 BX43 5 Adjusting the Tilt (Figs. 35 to 38) With the U-TBI-3 (Fig. 35) } Adjust the height and tilt of the observation tube to obtain the most comfortable viewing position. U-TBI-3 5° to 35° U-TBI-3-CLT 5° to 35° U-ETBI 0° ...

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24 Fig. 39 6 Using Eyepieces Incorporating a Micrometer } When the eyepieces in use incorporate a micrometer, the accuracy of the left-right focusing adjustment (diopter adjustment) can be improved further. 1. Looking into the right eyepiece wi...

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25 BX43 Fig. 40 Fig. 41 5-6 Immersion Objectives Be sure to use the provided Olympus Immersion oil. 1 Using Immersion Objectives (Fig. 40) 1. Focus on the specimen with objectives in the order of lower-power to higher-power ones. 2. Before ...

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26 6 CAMERA RECORDING } Use a trinocular observation tube such as the U-TR30-2 or U-SWTR-2 when shooting the video or digital camera picture of the microscope images. The trinocular tube accepts a camera adapter (certain TV adapters necessitate a...

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27 BX43 TROUBLESHOOTING GUIDE 7 Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve...

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28 Problem Lamp Cause Remedy Page Dirt or dust is visible in the field of view. Common Dirt/dust on the eyepieces. Clean thoroughly. 3 Common Dirt or dust on condenser top lens. Common Dirt/dust on the specimen. Visibility is poor · Image i...

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29 BX43 Problem Lamp Cause Remedy Page Coarse adjustment will not go all the way up. Common Pre-focusing lever is locked at a low position. Unlock pre-focusing lever. 17 Coarse adjustment will not go all the way down. Common Condenser holder is t...

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30 8 SPECIFICATIONS Item Specification 1. Optical system UIS2 (UIS) optical system (featuring infinity correction) 2. Illumination Built-in transmitted Koehler illumination FN (Field Number): 26.5 (super-widefield compatible) LED illuminator (contin...

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31 BX43 Item Specification 7. Condenser Type U-LC U-AC2 U-SC3 U-AAC Low magnification Abbe Swing-out Achromat/ Aplanat Max. N.A. 0.75 1.10 0.9 1.40 Applicable objective powers 2X - 60X (Widefield: FN 22) 4X (Widefield: FN 22) 10X - 100X (Super ...

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32 OPTICAL CHARACTERISTICS (UIS2 Series) 9 UIS series objectives not listed here can also be combined with this microscope. The following table shows the optical characteristics of com- binations of eyepieces and objectives. The figure on the righ...

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6 1 9 7 8 5 10 2 11 4 3 4 4 4 1212 33 BX43 ASSEMBLY 10 10-1 Assembly Diagram The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly. Assembly steps enclosed in wi...

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34 10-2 Detailed Assembly Procedures Fig. 42 Fig. 43 Fig. 44 Attaching the Auxiliary Lens for the U-LC Condenser 2 (Fig. 42) } It is not necessary to attach the auxiliary lens if the condenser in use is other than the U-LC. 1. Attach the auxi...

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35 BX43 Fig. 45 Fig. 46 Fig. 47 Fig. 48 8 Attaching the Revolving Nosepiece (Fig. 48) 1. Rotate the coarse adjustment knob to lower the stage. 2. Loosen the revolving nosepiece clamping screw @ slightly. Do not loosen it too much because doing ...

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36 Fig. 51 9 Attaching the Condenser (Fig. 50) 1. Turn the coarse adjustment knob @ to raise the stage to its upper limit. 2. Turn the condenser height adjustment knob 2 to lower the condenser holder to its lowest position. 3. Fully loosen the ...

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37 BX43 Fig. 52 Fig. 53 11 Attaching the AC Adapter · Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force. · Make sure that the main switch is set to “ ” (OFF) before connecting the power c...

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38 11 HALOGEN LAMP SOCKET INSPECTION SHEET { For safe use of the lamp socket, we recommend performing the following inspection periodically (every time you replace the lamp bulb and at least every 6 months). { The table below identified the check i...

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39 BX43 PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you us...

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40 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Printed or Embossed Harmoni- zation Marking (May be located on jacket or insulation of internal wiring) Alternative Mar...

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MEMO ...

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MEMO ...

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...

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Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstrasse 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, ...