Nikon E800 Operating Instructions Manual

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Nikon E800 Manual Information:

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Summary of Contents:

[Page 1] Nikon E800

!"#$%&#'&())*( ( ( ( +( Nikon E800 Operating Instructions Note: These instructions were written for the microscope, equipped with a Diagnostic Instruments SPOT camera, located in the Life Science Microscopy Facility at Purdue U...

[Page 2] Nikon E800

,( Turning on the Microscope: 1) Sign in on the log sheet. 2) Turn on the main power and the camera power. 3) Turn the microscope power to “on”. 4) Turn on the Fluorescence power only if you plan to use fluorescence imaging. Plea...

[Page 3] Nikon E800

:( Parts of the microscope: Neutral density filters to adjust illumination if required. They can be used in any combination to adjust the illumination so that it is comfortable for your eyes.. Push lever down to engage filter. Filters ar...

[Page 4] Nikon E800

A( Adjusting the microscope for Koehler illumination. 1) Insert a sample and focus it using an objective that you typically use for imaging. Tip: Focus on the edge of the coverslip to set the course focus. Then focus up and down using f...

[Page 5] Nikon E800

B( Taking an Image in Brightfield Mode: 1) Complete the Koehler alignment. Find an appropriate area of your sample and focus. 2) Open the SPOT Advanced program. 3) Select Brightfield-grayscale or brightfield-RGB in the lower right of the ...

[Page 6] Nikon E800

L( Adjusting the microscope for Nomarski DIC imaging 1) Select DIC grayscale or DIC color on the lower left of the SPOT camera program window. 2) Make sure the camera filter selector is pushed in for grayscale or pulled out for color. 3) ...

[Page 7] Nikon E800

P( 10) From the Setup menu, use “Setup > Image setup” to get into the dialogue boxes to adjust image. a. Select “modify” in Setups dialogue box. b. Select Exposure dialogue box and make sure “auto” is selected. It should a...

[Page 8] Nikon E800

S( Fluorescent Imaging 1. Align the microscope for Koehler Illumination as described above. 2. Locate your sample using either brightfield or Nomarski DIC imaging. 3. In most cases, the fluorescence signal is not bright enough to use camera...