WPI MF200 Instruction Manual

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[Page 1] WPI MF200

Instrumenting scientific ideas WORLD PRECISION INSTRUMENTS MF200 Microforge with 40× long working distance objective Serial No._____________________ www.wpiinc.com INSTRUCTION MANUAL 030716 ...

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MF200 World Precision Instruments i Copyright © 2016 by World Precision Instruments, Inc. All rights reserved. No part of this publication may be reproduced or translated into any language, in any form, without prior written permission of World P...

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ii World Precision Instruments ...

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MF200 World Precision Instruments 1 ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicate...

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2 World Precision Instruments INTRODUCTION The MF200 Microforge is a versatile device designed specically for the fabrication of glass micropipettes and other related tools. Originally designed by Dr. Ming Li of the Department of Pharmacology, ...

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MF200 World Precision Instruments 3 INSTRUMENT DESCRIPTION Parts List After unpacking, verify that there is no visible damage to the instrument. Verify that all items are included: MF200-1/2 Complete Microforge 110V/220V (includes microscope): (1) ...

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4 World Precision Instruments Description The complete MF200-1 (110V) and MF200-2 (220V) systems include both the Microforge and matched microscope (WPI Model W30S); the MF200–M1 (110V) and MF200-M2 (220V) include the Microforge only. Optics The...

[Page 9] WPI MF200

MF200 World Precision Instruments 5 Positioning and Focus Finding and moving the pipette tip under the microscope objective is simple. With a conventional microforge, it is dicult and time-consuming to position both the heating lament and pi...

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6 World Precision Instruments characteristic of the lament ensures minimal displacement of the lament during heating. This feature takes much of the guesswork out of tip placement in relation to the lament. Three functionally distinct hea...

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MF200 World Precision Instruments 7 push-button polish switch on the Control Unit turns the heating lament on and o. An optional foot switch is available for complex re polishing. Use of the optional foot switch leaves the hands free to m...

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8 World Precision Instruments OPERATING INSTRUCTIONS Setting up the MF200 Four steps are required to set up the MF200. First, mount the LWD to the microscope. Then, mount the Filament Adjustment Assembly to the microscope. Next, position the micro...

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MF200 World Precision Instruments 9 Mounting the Filament Adjustment Assembly to the Microscope 1. Using the 3/16 hex wrench provided, loosen the mounting screw on the Filament Adjustment Assembly to open the ring so that it will t comfortably...

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10 World Precision Instruments 4. Connect the appropriate plug from the AC/DC converter into the 12VDC receptacle on the Microforge Control Unit, and then plug the converter into a wall outlet. Positioning the Micropipette Holder Position the Pip...

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MF200 World Precision Instruments 11 Basic Operations for Using the MF200 This section describes the nal preparations and general instructions for using the MF200. Specic instructions are detailed for some of the MF200 common uses in “App...

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12 World Precision Instruments NOTE: The underside of the Microforge Control Unit and the AC/DC converter become warm to the touch during use. This is normal and no action is required. 6. Position the heating lament and the pipette: With th...

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MF200 World Precision Instruments 13 Table 2: Filament Power Switch Filament LARGE (or LOW) Power Position SMALL (or HIGH) Power Posi- tion H2 When polishing large pipette tips, the H2 lament works best. In most cases LOW power will per- form s...

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14 World Precision Instruments Applications Choice of laments, power and heat settings for each application vary with the use. If the desired result is achieved, the choice of parameters is acceptable. Always use the least amount of heat possib...

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MF200 World Precision Instruments 15 Step 2: Fire Polish the Single-Channel and Whole Cell Patch Clamp Pipette 1. Choose and install the desired lament. (See “Table 2: Filament Power Switch” on page 13.) 2. Turn on the Control Unit power....

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16 World Precision Instruments Tip Size Reduction Tip size reduction creates a holding pipette by rounding the tip ends and reducing the length of the pipette tip (Fig. 11). Fig. 11—(Right) Tip size reduction 1. Choose and install the desired �...

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MF200 World Precision Instruments 17 Tip” on page 15. Tip Reduction of Large Bore Pipettes The re-formed H2 lament described in “Fire Polishing Large Bore Pipettes” can also be used for tip size reduction of large bore pipettes. To reshap...

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18 World Precision Instruments 2. Adjust the microscope stage until the pipette is in position to allow the tip to touch the lament during expansion. 3. Set the dial so the lament starts to glow red. Press Polish and coat the center of th...

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MF200 World Precision Instruments 19 INSTRUMENT MAINTENANCE The requirements for the maintenance and storage of the MF200 are minimal. Care should be taken to protect the laments. Store them in their original container when not in use. In gener...

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20 World Precision Instruments SPECIFICATIONS AC POWER MODULE 100-240 VAC 50/60Hz FILAMENTS (3) H2, H3, H4 FILAMENT “ON” CONTROL Push button or Optional Foot Switch FILAMENT ADJUSTMENT ASSEMBLY Mounts on 40× and 25× Long-Working Dist...

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MF200 World Precision Instruments 21 INDEX A accessories 19 air supply 14 applications 14 B base plate 9 burn out 12 C changing laments 12 control unit 6 F Filament Adjustment Assembly 3, 5, 6 mounting 8, 9 lament clamp 9, 10...

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22 World Precision Instruments DECLARATION OF CONFORMITY ...

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MF200 World Precision Instruments 23 * Electrodes, batteries and other consumable parts are warranted for 30 days only from the date on which the customer receives these items. WARRANTY WPI (World Precision Instruments, Inc.) warrants to the origin...

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USA International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: [email protected] UK 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 42470...