Zeiss AxioImager A1 Basic Operation Instructions

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[Page 1] Zeiss AxioImager A1

Instructions for Zeiss AxioImager A1 Microscope for Stereology Basic operation instructions as of 10/1/2009 The Stereology Workstation By Jon Ekman ...

[Page 2] Zeiss AxioImager A1

Start-up 1. Turn MASTER switch on Belkin Surge- Master. This will turn on the LUDL stage controller, microscope stand and the Olympus Microre color CCD camera. 2. Turn ON X-Cite 120 Hg lamp if needed. Note: The X-Cite 120 must stay ON a minim...

[Page 3] Zeiss AxioImager A1

Align Optical Components Koehler Illumination PROCEDURE: Transmitted Light 1. Focus on specimen with the objective to be used for data collection. 2. Close down the luminous eld dia- phragm (1) while viewing. 3. Raise or Lower condenser vertical ...

[Page 4] Zeiss AxioImager A1

Calibration The system is calibrated by ITG sta, and new users get these initial calibration settings at rst login and startup. The calibration is done using transmit- ted brighteld method. Calibrate only the objectives you plan to use ...

[Page 5] Zeiss AxioImager A1

Stereology with the Optical Fractionator Workow Tissue Preparation The optical fractionator requires fairly thick tissue. Thicker tissue allows one to distinguish between cells that are on top of each other while focusing through the tissue m...

[Page 6] Zeiss AxioImager A1

Optical Fractionator Workow Continued... 100x-oil). Objective choice will depend on tissue type and staining. Note: objec- tives with magnication lower than 40x will not give you enough depth of view to count accurately especially when cell...

[Page 7] Zeiss AxioImager A1

DO NOT save each section as a new le. Use the workow to add new sections, as described above, until all the sections in an animal are traced and counted. NOTE: One animal = one le. and past the bottom of the tissue until it is complet...

[Page 8] Zeiss AxioImager A1

Start-up . . . . . . . . . . . . . 1 . . . . . . . . . Turn ON Master Switch 2 . . . . . . . . . . . Log on to Computer 3 . . . . . . . . . . . . . Load...

[Page 9] Zeiss AxioImager A1

Acquire Images Objective List All require 0.17mm coverslip unless noted 2.5x . . . . . . . .Plan-Neouar 1 NA . . . . . . . . . . . . . . . . . 0.075 WD(mm) . . . . . . . . . . ...

[Page 10] Zeiss AxioImager A1

Saving les 1. Save to Local Machine. --Default You may save les to Workspace (E:\Workspace\”username”). A folder was created there at login for each user. 2. Before leaving, save to Network Share -- “username” on ‘ITG File Server (...

[Page 11] Zeiss AxioImager A1

References Calibration Standards Calibration Grid Slide from Micro Brighteld: http://www.mbfbioscience.com Fluorescent Reference Slides from Micros- copy Education: http://www.microscopyeducation.com Olympus http://www.olympusamerica.com/seg_sec- ...